ISU REPORT ON ITS SCIENTIFIC-RESEARCH ACTIVITY –INSTITUTE OF CHEMICAL BIOLOGY
On December 14, at 17:00, at room E207 of Ilia State University (ISU), within the framework of the report on scientific-research activity of ISU, the presentation on scientific-research projects will be held by the Institute of Chemical Biology of the University.
The presentation of 2 scientific-research projects will be conducted.
The report on the topic Molecular Mechanisms of Recognition Memory of Imprinting in Chickens will be given by Prof. Revaz Solomonia.
The prime difficulty in perceiving molecular bases of memorization and memory is an identification of those areas of the brain that participate in the processes of preservation. This difficulty has mainly been surmounted for visionary imprinting that constitutes the process of memorization during which newly-born domestic chickens learn and later decipher the characteristics of an object in question. Before imprinting, no visionary memorization has any effect on the brain. There exists firm overlapping data that enclosed area of frontal lobe, intermediate and medial mesopalium (IMM) keeps the information from imprinting and other memorization process (1). Through this model, it becomes possible to correlate quantitative-behavioral figures to quantitative figures of biochemical changes of the area. On the basis of the priority of these qualities it became possible to determine specific series of molecular changes incorporating different cellular processes. The report will focus on specific changes for memorization and memory in microRNAs, epigenetic changes of chondriosome DNM, etc.
A microRNA (abbreviated miRNA) is a small non-coding RNA molecule (containing about 22 nucleotides) found in plants, animals and some viruses that functions in RNA silencing and post-transcriptional regulation of gene expression. We compared changes in a microRNA spectrum, after 24 hours from the act of memorizing, in the IMM of the left hemisphere of chickens with a good and bad memorizing ability. These comparison revealed 12 microRNAs the expression of which is considerably different between the groups. For further validation, from microRNAs, was chosen mir-130b, which is characterized by average frequency expression. We demonstrated specific declension for mir-130b memory in the left IMM.
The report on the topic Engagement of Metabotropic Glutamate Receptor 5 in Macrophage Plasticity will be given by Lali Shanshiashvili.
Macrophages play a significant role in protecting hosts and preserving homeostasis. Functional macrophages are heterogenic cells and, according to their dependence on microenvironment, are polarized in two groups: classically activated M1 and alternatively activated M2 phenotypes. Glutamate acid and glutamate receptors may be involved in the plasticity of macrophages. We carried out transfection RAW-264.7 of metabotropic glutamate receptor 5 in macrophagic cells. Comparative analysis of transfected and non-transfected cells demonstrated that transfected macrophages adsorb more glutamate that controlling cells. Intercellular concentration of glutamate correlated with the expression of transporter 2 of cationic amino acids. In addition, in transfected macrophages the expression of receptor-g increased.
We assume that the increase of concentration of intercellular glutamate and glutamate metabotropic receptor 5 can cause metabolic rearrangement of macrophages and formation of immunosuppressive M2 phenotype.
Date and Time: December 14, 17:00
Venue: E 207 room, Cholokashvili Ave., ISU
All are welcomed to attend the event