ISU REPORT ON ITS SCIENTIFIC-RESEARCH ACTIVITY –INSTITUTE OF BIOPHYSICS
On December 13, at 17:00, at Ilia State University (ISU) E 207 room, (Cholokashvili Ave., #3/5), within the framework of the report on scientific-research activity of ISU, the presentation on scientific-research projects will be held by the Biophysics Institute of the University.
The presentation of two scientific-research projects will be held.
The report on the topic Quadruplex Priming Amplification (QPA) for Molecular Diagnostics will be given by Levan Lomidze.
Quadruplex priming amplification (QPA) constitutes a method which allows isothermal amplification of DNA and possesses intrinsic real-time detection mechanism. The key mechanism of QPA is DNA pattern GGGTGGGTGGGTGGG (G3T), which forms strong thermal stability structure Quadruplex. G3T, which in its 3’-praim end lacks guanine, is used as a primer in the reaction. Missing guanine residues is critical for quadruplex formation, and since it is missing quadruplex cannot be formed. However, when polymers add guanine to primer, GST folds into a quadruplex in the wake of what primer, in an isothermal way, is separated from the target DNM, and the primer end is free to attach the next primer. Also primers incorporate fluorescent nucleotide, e.g. 3-methylisoxanthopterin, which is characterized by amplifying fluorescent signals after the formation of quadruplex, and this proves to be an effective means for determining signals without fluorescent signaling molecules. We created QPA reaction, which allows an effective, fast and isothermal amplification of DNM; the aforementioned method can widely be applied to discover biomedical, molecular biology, genetics and forensic science diseases and pathogenic microorganisms, as well as in scientific research.
The report on the topic Laser-induced Fluorescent Resonance Energy Transfer to Study Defects in DNM Dual Spiral will be delivered by Irina Khutsishvili.
Fluorescent resonance method for transferring nanoscale energy allows to determine concentration of DNM dual spiral areas that is useful for intercalation. This method enables us to hold DNM comparative analysis in different damaged DNM structures. Through Fluorescence Resonance Energy Transfer Method (FRET) has been studied DNM complexes with recovered silver and copper ions and silver particles; the effect of temperature on DNM dual spiral has also been explored.
Date and Time: 13 December, 17:00
Venue: E 207 room (Cholokashvili Ave., #3/5), ISU
All are welcomed to attend the event.