ILIA STATE UNIVERSITY

Public Advertisement of Procurement

Public Advertisement of Procurement: the CaBOL Project is procuring the Laboratory Equipment

Illumina MiniSeq
Specifications:

  • Sequencing with read length 1x 75 bp, 2x 75bp, 2x 150bp
  • Sequencing chemistry base by base incorporation with LED light detection 515 nm and 650 nm wave length
  • Ability to perform clonal amplification, sequencing, paired-end turn
  • Single-run scalability (2-7.5 Gb) to support a broad range of applications and study sizes
  • Sequencing runs complete in ~4-24 hours
  • Instrument should employ high-accuracy 2-channel SBS (sequencing by synthesis) technology
  • Accurate sequencing of homopolymers
  • Fully automated paired-end sequencing
  • Alignment, variant calling and reporting in a modern genomics computing environment
  • Instrument footprint requires less an 2 square feet of benchtop space
  • Exceptionally high-quality score distributions of >80% of bases with Q-scores >30 (2x150 bp)
    • Fully automated onboard cluster generation enabling direct loading of prepared libraries onto the instrument
    • Fully automated on-board data analysis including base calling, alignment, variant calling and reporting with LRM apps, output data should be compatible with LRM (Local Run Manager) software
    • Instrument control computer is integrated in the sequencer, processing power at least equivalent to i7- 4700EQ 2.4 GHz CPU with 16 Gb DDR3L RAM for instrument control, processing images, base calling and further analysis
    • Instrument produces FASTQ, BAM, VCF and txt formatted files for maximum compatibility with third-party downstream software packages
      • Applications:
        • Small whole-genome resequencing
        • Targeted sequencing including but not limited to the methods: exomes (37-62 Mb), custom enrichment panels (100s kb-Mbs), custom amplicon panels (100s kb)
        • De novo sequencing
        • Mate pair sequencing for libraries with 2-5 kb insert sizes
        • ChIP-Seq of sequence-specific DNA binding proteins
        • mRNA sequencing
          • Tag-based gene expression
          • Small RNA sequencing
          • Targeted RNA sequencing
          • DNase 1 hypersensitivity site mapping
          • ChIP-Seq: studying sequence-specific protein-RNA interactions
          • CNV-Seq: measuring copy number variation (CNV) with sequencing
          • Paired-end mRNA sequencing to study gene fusions in cancer
          • Sequencing of ancient DNA samples
          • DNA imprinting and allele-specific expressions
            • 16S metagenomics sequencing

Companies and suppliers are invited to send their bids to email address: purchase@iliauni.edu.ge

Only bids for factory-new equipment are accepted and suppliers should state guaranteed means of technical support in installation and maintainance as well as the ability to supply spare parts if needed."

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